α-Solanine Suppresses Glioblastoma Multiforme Growth and Metastatic Properties by Modulating the Apoptosis-autophagy Axis

Introduction: Glioblastoma multiforme (GBM) has a poor prognosis despite optimal treatment. Recent studies have shown the potential of phytochemicals as anti-cancer agents. α-solanine, derived from plants Solanum genus, is promising molecule in this regard. This study investigated efficacy α-solanine compared to temozolomide (TMZ) against GBM cell lines (U87MG, U251, and T98G) vitro.
 Methods: In-vitro assays were conducted assess viability, migration, invasion, mode death U87MG, U251 T98G following treatment comparison TMZ. Rt-qPCR proteome profiling investigate changes induced by on molecular level.
 Results: demonstrated potent cytotoxicity all lines, with IC50 values ranging 19.66 µM 22.87 between tested, significantly inhibited migration TMZ RNA protein level indicated upregulation both apoptotic autophagy proteins, suggesting involvement BECN1 BNIP3L α-solanine-induced death.
 Discussion Conclusion: These findings contribute search for effective treatments. Future should increase number biological replicates, employ alternative methods strengthen findings, conduct vivo experiments testing using patient-derived tissue better evaluate any therapeutic suitability fully understand action GBM.